All terms
Limit of Detection / Limit of Quantitation
Lowest analyte concentrations an assay can reliably detect (LoD) or quantify (LoQ).
Reviewed by Christian Espinosa, Founder, Blue Goat CyberLast reviewed May 5, 2026
Definition
LoD is the lowest concentration distinguishable from a blank with stated probability. LoQ is the lowest concentration that can be quantitatively measured with stated accuracy and precision (per CLSI EP17 / EP05).What this means in practice
Critical performance characteristics for IVD claims, especially for infectious disease and oncology assays where low-burden detection drives clinical utility.Cross-references
See also
Closely related context worth reading.
Primary references
3 sourcesLink health: 3 verified· last checked 2026-05-09
CLSI·1ISO·1FDA·1
- 1
CLSI EP17VerifiedCLSIclsi.org
- 2
ISO 14155 Standard PageVerifiedISOiso.org
- 3
FDA - Clinical Trials and Human Subject ProtectionVerifiedFDAfda.gov
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